|Immunocytochemistry (ICC)||10 µg/ml|
|Immunofluorescence (IF)||20 µg/ml|
|Western Blot (WB)||0.5 µg/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids near the carboxy terminus of human ASK1|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
In Western blot applications, this antibody detects a band at ~155kDa. A suggested positive control is SW1353 cell lysate.
PA5-19880 can be used with blocking peptide PEP-0255.
Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-a-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: apoptosis signal regulating kinase 1; Apoptosis signal-regulating kinase 1; ASK-1; MAP/ERK kinase kinase 5; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5; Mitogen-activated protein kinase kinase kinase 5
Gene Aliases: ASK1; MAP3K5; MAPKKK5; MEKK5
UniProt ID: (Human) Q99683
Entrez Gene ID: (Human) 4217
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