|Western Blot (WB)||2 µg/ml|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 1-100 of mouse ATG16L1. [Swiss-Prot# Q8C0J2]|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 30% glycerol|
|Contains||0.1% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
Suggested positive control: NIH-3T3 whole cell lysates.
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The APG12-APG5-APG16L complex is essential for the elongation of autophagic isolation membranes. This complex initially associates in uniform distribution with small vesicle membranes. During membrane elongation, the complex partitions, with a great concentration building on the outer side of the isolation membrane. Upon completion of the formation of the autophagosome, the APG12-APG5-APG16L dissociates from the membrane.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: apg16 autophagy 16like s cerevisiae; APG16-like 1; APG16L beta; ATG16 autophagy related 16-like 1; atg16l anti; Atg16L1 gamma; autophagy related 16-like 1; autophagy-related 16-like 1 gamma; Autophagy-related protein 16-1; WD repeat domain 30; wdr30
Gene Aliases: 1500009K01Rik; APG16L; ATG16A; ATG16L; ATG16L1; IBD10; UNQ9393/PRO34307; WDR30
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