|Flow Cytometry (Flow)||3-5µg/million cells|
|Western Blot (WB)||1-2µg/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||full-length protein corresponding to Human ATG4b|
|Contains||0.09% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
This antibody is predicted to react with monkey, mouse, rat, guinea pig and bovine based on sequence homology.
ABfinity™ recombinant oligoclonal antibodies are rabbit antibody pools generated from isolated DNA libraries. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ oligoclonal antibodies recognize multiple epitopes and therefore exhibit greater sensitivity and because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Cysteine protease ATG4B is an enzyme which is coded by ATG4B gene. Autophagy is a critical cellular homeostatic process that controls the turnover of damaged organelles and protein. ATG4B is a deubiquitin-like protease that plays dual roles in the core machinery of autophagy. ATG4B, cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. ATG4B is mainly expressed in the skeletal muscle, brain, heart, liver and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: APG4 autophagy 4 homolog B; ATG4 autophagy related 4 homolog B; AUT-like 1 cysteine endopeptidase; AUTL1; Autophagin-1; autophagy related 4B, cysteine peptidase; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; Cysteine protease ATG4B; hAPG4B
Gene Aliases: APG4B; ATG4B; AUTL1; KIAA0943
UniProt ID: (Human) Q9Y4P1
Entrez Gene ID: (Human) 23192
Molecular Function: hydrolase
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