|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:400|
|Western Blot (WB)||2 µg/ml|
|Tested Species reactivity||Bovine, Human, Mouse, Non-human primate, Pig, Rat, Xenopus, Zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide made to an N-terminal region of the human ATG5 protein.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
In Western Blot, a band is seen ~56 kDa representing the ATG5-ATG12 complex, the molecular weight of human ATG5 is ~33 kDa.
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Suggested positive control: antigen standard for ATG5 (transient overexpression lysate), mouse wildtype ES cell lysate.
Autophagy is an important mechanism for nonselective intracellular breakdown whereby cytosol and organelles are encapsulated in vesicles, which are then engulfed and digested by lytic vacuoles/lysosomes. ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediate before or after autophagosomes formation is completed. Conjugation to ATG12 is essential to autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the Apoptotic process. It's expression is a relitively late event in the apoptotic process, occuring down stream of caspase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 2010107M05Rik; 3110067M24Rik; APG5-like; Apg5l; Apoptosis-specific protein; ATG5 autophagy related 5 homolog; Atg5l; autophagy 5-like protein; Autophagy protein 5; autophagy related 5; autophagy-related 5; AW319544; C88337; Paddy
Gene Aliases: 2010107M05Rik; 3110067M24Rik; APG5; APG5-LIKE; APG5L; ASP; ATG5; Atg5l; AW319544; BOS_9664; C88337; hAPG5; Paddy; zgc:100934
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.