|Immunocytochemistry (ICC)||20 ug/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Immunohistochemistry (IHC)||2.5 ug/ml|
|Western Blot (WB)||1-2 ug/ml|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 16 amino acid peptide from near the amino terminus of human ATG5.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
A suggested positive control is rat spleen tissue lysate.
PA5-20484 can be used with blocking peptide PEP-0604.
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG5, another member of the autophagy protein family, forms a conjugate with ATG12; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: APG5, APG5-LIKE, APG5L, ASP, hAPG5; APG5-like; apoptosis specific protein; Apoptosis-specific protein; ATG5 autophagy related 5 homolog; Autophagy protein 5; autophagy-related 5
Gene Aliases: 2010107M05Rik; 3110067M24Rik; APG5; APG5-LIKE; APG5L; ASP; ATG5; Atg5l; AW319544; C88337; hAPG5; Paddy
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