|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||1:500|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Armenian hamster / IgG|
|Immunogen||peptide sequence HPEPVPEEGSEDELPPQVHK of human ATG9A C-terminus|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
MA1-149 was produced in Armenian hamster and detects ATG9 in human, rat and mouse samples. MA1-149 has been successfully used in Western Blot, Immunofluorescence, and ELISA procedures. Western Blot analysis with MA1-149 shows the detection of a double band at ~85-95 kDa in human ATG9 overerexpression lysates. MA1-149 also detects additional unknown bands at ~40 and ~60 kDa. In Immunofluorescence applications, MA1-149 shows accumulation and redestribution of ATG9 in response to starvation-induced autophagosome assembly.
Autophagy is an important process associated with infectious disease, neurodegeneration, cancer and the maintenance of cellular homeostasis. ATG9 is an essential component of the autophagy machinery and is found on autophagosomes. ATG9 forms a cycling complex with ATG2 and ATG18, which is thought to constantly deliver lipids from source to growing autophagosomes during early autophagosome assembly. ATG9 is a multi-spanning membrane protein and cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. The detection of this protein provides important information about cell health and survival.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: APG9 Autophagy 9-Like 1; APG9-Like 1; APG9L1; ATG 9A; ATG9 Autophagy Related 9 Homolog A; autophagy 9-like 1 protein; autophagy protein 9; Autophagy Related 9A; autophagy-related 9-like 1; autophagy-related 9A; Autophagy-related protein 9A; FLJ22169; mATG9; MGD3208
Gene Aliases: APG9L1; Atg9; ATG9A; Atg9l1; AU019532; mATG9; MGD3208; RGD1310450
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