|Flow Cytometry (Flow)||1 µg/ml per 10^6 cells|
|Immunocytochemistry (ICC)||5 ug/ml|
|Immunofluorescence (IF)||5 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Western Blot (WB)||1-2 ug/ml|
|Western Blot (WB)||See 13 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Tested Species reactivity||Bovine, Human, Mouse, Rat, Zebrafish|
|Published species reactivity||Rat , Bovine , Mouse , Human|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Bovine Complex V|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage conditions||4° C, do not freeze|
Use Isolated mitochondria from Human, Rat, Bovine and Mouse Heart, and HepG2, Cultured Human embryonic lung-derived fibroblasts (strain MRC5), HL-60 cells for positive controls.
ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.
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Protein Aliases: alpha subunit; ATP synthase; ATP synthase alpha chain; ATP synthase alpha chain, mitochondrial; ATP synthase alpha subunit; ATP synthase subunit alpha; ATP synthase subunit alpha, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2; ATP sythase (F1-ATPase) alpha subunit; ATP5A; ATP5AL2; ATPM; cardiac muscle; epididymis secretory sperm binding protein Li 123m; H+ transporting; hATP1; iso; isoform 1; mitochondrial; mitochondrial ATP synthase alpha subunit; mitochondrial ATP synthetase, oligomycin-resistant; mitochondrial F1 complex; mitochondrial H+-ATP synthase alpha subunit; modifier of Min 2; MOM2; OMR; ORM
Gene Aliases: AI035633; AL022851; AL023067; ATP5A; ATP5A1; ATP5A2; ATP5AL2; ATPM; BOS_22238; COXPD22; D18Ertd206e; hATP1; HEL-S-123m; MC5DN4; MOM2; OMR; ORM; zgc:154103
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