|Flow Cytometry (Flow)||1:10-1:50|
|Western Blot (WB)||1:1000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 44-71 amino acids from the N-terminal region of human ATP6V0A1|
|Purification||Antigen affinity chromatography|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
This antibody is predicted to react with bovine, chicken, mouse, rat and Xenopus based on sequence homology.
ATP6V0A1 encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes one of three A subunit proteins and the encoded protein is associated with clathrin-coated vesicles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 116 kDa accessory protein A1; a1; ATP6N1; ATP6N1A; ATPase; ATPase, H+ transporting, lysosomal non-catalytic accessory protein 1 (110/116kD); ATPase, H+ transporting, lysosomal V0 subunit a1; Clathrin-coated vesicle/synaptic vesicle proton pump 116 kDa subunit; H(+)-transporting two-sector ATPase; H(+)-transporting two-sector ATPase, 116 kDa accessory protein A1; H+ transporting; lysosomal (vacuolar proton pump) non-catalytic accessory protein 1A (110/116kD); lysosomal non-catalytic accessory protein 1 (110/116kD); Stv1; V-A; V-ATPase 116 kDa; V-ATPase 116 kDa isoform a1; V-type proton ATPase 116 kDa subunit a; V-type proton ATPase 116 kDa subunit a isoform 1; Vacuolar adenosine triphosphatase subunit Ac116; Vacuolar proton pump subunit 1; vacuolar proton translocating ATPase 116 kDa subunit A; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 1; vacuolar-type H(+)-ATPase 115 kDa subunit; Vph1; VPP1
Gene Aliases: a1; ATP6N1; ATP6N1A; ATP6V0A1; Stv1; Vph1; VPP1
UniProt ID: (Human) Q93050
Entrez Gene ID: (Human) 535
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