|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 53 and 333 of Human ATP6V0A4|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-29109 targets ATP6V0A4 in IF and WB applications and shows reactivity with Human samples.
The PA5-29109 immunogen is recombinant fragment corresponding to a region within amino acids 53 and 333 of Human ATP6V0A4.
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. This gene is one of four genes in man and mouse that encode different isoforms of the a subunit. Alternatively spliced transcript variants encoding the same protein have been described. Mutations in this gene are associated with renal tubular acidosis associated with preserved hearing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ATP6N1B; ATP6N2; ATPase, H+ transporting, lysosomal (vacuolar proton pump) non-catalytic accessory protein 1B; ATPase, H+ transporting, lysosomal (vacuolar proton pump) non-catalytic accessory protein 2 (38kD); ATPase, H+ transporting, lysosomal V0 subunit a4; H(+)-transporting two-sector ATPase; H(+)-transporting two-sector ATPase, noncatalytic accessory protein 1B; H+ transporting; lysosomal (vacuolar proton pump) non-catalytic accessory protein 1B; lysosomal (vacuolar proton pump) non-catalytic accessory protein 2 (38kD); noncatalytic accessory pro; RDRTA2; RTA1C; RTADR; STV1; V-ATPase 116 kDa; V-ATPase 116 kDa isoform a4; V-type proton ATPase 116 kDa subunit a; V-type proton ATPase 116 kDa subunit a isoform 4; vacuolar proton pump 116 kDa accessory subunit; vacuolar proton pump, subunit 2; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 4; Vacuolar proton translocating ATPase 116 kDa subunit a kidney isoform; VPH1; VPP2
Gene Aliases: A4; ATP6N1B; ATP6N2; ATP6V0A4; RDRTA2; RTA1C; RTADR; STV1; VPH1; VPP2
UniProt ID: (Human) Q9HBG4
Entrez Gene ID: (Human) 50617
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