|ChIP assay (ChIP)||10 µg|
|Dot blot (DB)||Assay dependent|
|Immunocytochemistry (ICC)||1:100 - 1:500|
|Immunofluorescence (IF)||1:100 - 1:500|
|Immunoprecipitation (IP)||3 µg|
|Western Blot (WB)||1:500 - 1:2000|
|Immunoprecipitation (IP)||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Western Blot (WB)||See 2 publications below|
|Tested Species reactivity||Chemical|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG|
|Immunogen||Synthetic peptide sequence surrounding the acetylated lysine 9 of histone H3.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-2021 detects human and mouse, and monkey acetylated lysine.
MA1-2021 has been used successfully in Western blot, immunoprecipitation, dot blot, ChIP Assay, and immunofluorescence (cells) procedures. By Western blot, this antibody may detect a ladder of acetylated proteins in HeLa cell lysate. In immunofluorescence procedures, MA1-2021 recognizes acetylated lysine in gamma irradiated HeLa cells.
The MA1-2021 immunogen is a synthetic peptide sequence surrounding the acetylated lysine 9 of histone H3.
In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Acetylated Lysine; Acetyllysine
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