|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||1 ug/ml|
|Immunofluorescence (IF)||1 ug/ml|
|Western Blot (WB)||1-3 ug/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide corresponding to amino acids 377–386 of human p53 [AcK382]|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This antibody is predicted to react with mouse, rat, non-human primate and rabbit based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 acetylation at Lys382 is required for recruitment of p300 to the p21 promoter, and is mediated by p300 and CBP acetyltransferases. Acetylation appears to play a positive role in the accumulation of p53 protein in stress responses, and inhibition of deacetylation stabilizes p53.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Antigen NY-CO-13; Cellular tumor antigen p53; FLJ92943; LFS1; mutant tumor protein 53; p53 tumor suppressor; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein 53; Tumor suppressor p53; tumor supressor p53
Gene Aliases: BCC7; LFS1; P53; TP53; TRP53
UniProt ID: (Human) P04637
Entrez Gene ID: (Human) 7157
Molecular Function: transcription factor
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