|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Western Blot (WB)||1 ug/ml|
|Immunohistochemistry (IHC)||See 9 publications below|
|Immunocytochemistry (ICC)||See 2 publications below|
|Tested Species reactivity||Dog, Chicken, Cat, Human, Mouse, Non-human primate, Rabbit, Rat|
|Published species reactivity||Human Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||SDS extracted protein fraction of human myocardium|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage conditions||4° C, do not freeze|
This antibody recognizes muscle-specific alpha and gamma actin (42 kDa) in various species. This antibody stains skeletal, smooth and myocardial cells as well as myoepithelial cells and pericytes of small vessels. It is a widely used marker of muscle and muscle-derived cells.
Actin is a highly conserved ubiquitous globular protein (G-actin) that polymerizes to form fibrous F-actin microfilaments. In higher eucaryotes several actin isoforms have been identified, that fall into three classes. Alpha actin is a structural component of the contractile apparatus of muscle cells or muscle-derived cells. beta actin and gamma actin play roles in regulation of cell motility in other cell types. Specific subcellular structures such as as stress fibers, focal adhesions, filopodia etc., are formed by involvement of actin cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: a-actin; ACTA; actin; actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; Actin, alpha cardiac muscle 1; Actin, alpha skeletal muscle; actin, alpha, cardiac 1; actin, alpha, cardiac muscle 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; Actin, cytoplasmic 2; actin, gamma, cytoplasmic 1; alpha skeletal muscle; alpha skeletal muscle actin; Alpha-actin; alpha-actin cardiac; Alpha-actin-1; Alpha-actin-2; Alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; ASMA; beta-actin FE-3; cardiac actin; Cell growth-inhibiting gene 46 protein; CFTD; CFTD1; CFTDM; cytoskeletal gamma-actin; deafness, autosomal dominant 20; deafness, autosomal dominant 26; epididymis luminal protein 176; Gamma-actin; MPFD; NEM1; NEM2; NEM3; nemaline myopathy type 3; RP5-1068B5.2; smooth muscle alpha-actin
Gene Aliases: 0610041G09Rik; a-SMA; AA959943; AAT6; ACT; ACTA; Acta-2; ACTA1; ACTA2; ACTC; Actc-1; ACTC1; ACTG; ACTG1; Actl; Acts; ACTSA; Actsk-1; ACTVS; AL023024; alphaSMA; ASD5; ASMA; BRWS2; CFTD; CFTD1; CFTDM; CMD1R; CMH11; DFNA20; DFNA26; E51; GIG46; HEL-176; LVNC4; MPFD; MYMY5; NEM1; NEM2; NEM3; SHPM; SMalphaA
UniProt ID: (Human) P68133, (Chicken) P68139, (Human) P62736, (Rabbit) P62740, (Chicken) P08023, (Human) P68032, (Chicken) P68034, (Human) P63261, (Rat) P68136, (Mouse) P68134, (Rat) P62738, (Mouse) P62737, (Mouse) P68033, (Rat) P68035, (Mouse) P63260
Entrez Gene ID: (Human) 58, (Chicken) 421534, (Human) 59, (Rabbit) 100009271, (Chicken) 423787, (Human) 70, (Rabbit) 100340339, (Dog) 478250, (Chicken) 423298, (Human) 71, (Rat) 29437, (Mouse) 11459, (Rat) 81633, (Mouse) 11475, (Mouse) 11464, (Rat) 29275, (Mouse) 11465, (Rat) 287876
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.