|Western Blot (WB)||1:5000-1:15,000|
|Western Blot (WB)||See 7 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Human , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||N-terminus of human beta actin using the numbering given in Swiss-prot entry P60709 and is highly conserved with gamma actin|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris citrate/tris phosphate, pH 7-8|
|Contains||0.09% sodium azide|
|Storage conditions||4° C, do not freeze|
This antibody will not work in ICC/IF applications.
Suggested positive control: Hela whole cell extract.
In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma, have been identified. The alpha actins are found in muscle tissues and are a major constituent of contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are hightly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Each actin can bind to 4 others. This antibody serves as an excellent loading control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: actin, beta; Actin, cytoplasmic 1; Atcb; beta actin; beta cytoskeletal actin; Beta-actin; cytoskeleton actin; gamma actin; PS1TP5-binding protein 1
Gene Aliases: ACTB; Actx; beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1
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