|ELISA (ELISA)||Assay dependent|
|Western Blot (WB)||1:1,000|
|ChIP assay (ChIP)||See 1 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Western Blot (WB)||See 72 publications below|
|Miscellaneous PubMed (MISC)||See 3 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Bovine, Fruit fly, Human, Mouse, Sheep, Plant, Rat, Xenopus, Yeast, Zebrafish|
|Published species reactivity||Yeast , Rat , Pig , Bacteria , Plant , Mouse , Human , Xenopus|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified Arabidopsis actin protein.|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-744 detects actin protein in human, mouse, rat, bovine, sheep, drosophila, zebra fish (Danio rerio), yeast, arabidopsis and xenopus samples. This antibody has shown cross-reactivity with actin 1, 2, 3, 4, 7, 8, 11 and 12.
MA1-744 has successfully been used in Western blot and ELISA procedures. By Western blot, this antibody detects a 45 kDa protein representing actin.
The MA1-744 immunogen is purified actin protein from Arabidopsis.
Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ACTA; ACTA1; actin alpha 1; Actin, alpha skeletal muscle; actin, alpha, cardiac muscle 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; actin, beta; Actin, cytoplasmic 1; alpha Actin 1; alpha actin 2; alpha skeletal muscle Actin; Alpha-actin-1; Alpha-actin-2; alpha-cardiac actin; alpha-sma; ASMA; Atcb; beta cytoskeletal actin; Beta-actin; Cell growth-inhibiting gene 46 protein; cytoplasmic beta-actin; NEM1; NEM2; nemaline myopathy type 3; PS1TP5-binding protein 1; smooth muscle alpha-actin
Gene Aliases: 0610041G09Rik; a-SMA; AA959943; AAT6; ACTA; Acta-2; ACTA1; ACTA2; ACTB; Acts; ACTSA; Actsk-1; ACTVS; Actx; alphaSMA; ASMA; beta-actin; BOS_22932; BOS_23286; BOS_23966; BRWS1; CFTD; CFTD1; CFTDM; E430023M04Rik; GIG46; MPFD; MYMY5; NEM1; NEM2; NEM3; PS1TP5BP1; SHPM; SMalphaA
UniProt ID: (Human) P68133, (Bovine) P68138, (Human) P62736, (Bovine) P62739, (Sheep) P60713, (Human) P60709, (Bovine) P60712, (Rat) P68136, (Mouse) P68134, (Rat) P62738, (Mouse) P62737, (Rat) P60711, (Mouse) P60710
Entrez Gene ID: (Human) 58, (Bovine) 281592, (Human) 59, (Bovine) 515610, (Sheep) 443052, (Human) 60, (Bovine) 280979, (Sheep) 101111554, (Rat) 29437, (Mouse) 11459, (Rat) 81633, (Mouse) 11475, (Rat) 81822, (Mouse) 11461, (Xenopus) 398459
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