|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1-3 µg/ml|
|Immunohistochemistry (IHC)||See 3 publications below|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Bovine, Dog, Horse, Human, Mouse, Pig, Rat|
|Published species reactivity||Fish , Zebrafish Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
This antibody reacts with alpha-smooth muscle, alpha-skeletal and alpha-cardiac (sarcomeric). This pan muscle actin antibody reacts with tumors arising from smooth muscle (leiomysarcomas) as well as skeletal muscle tumors (rhabdomyosarcomas).
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: actin; actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; Actin, alpha cardiac muscle 1; Actin, alpha skeletal muscle; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; alpha actin 1; alpha actin 2; alpha skeletal muscle; alpha skeletal muscle actin; alpha-actin cardiac; Alpha-actin-1; Alpha-actin-2; Alpha-cardiac actin; alpha-sma; alphac-actin; Cell growth-inhibiting gene 46 protein; nemaline myopathy type 3; skeletal alpha actin; smooth muscle alpha-actin
Gene Aliases: 0610041G09Rik; a-SMA; AA959943; AAT6; ACTA; Acta-2; ACTA1; ACTA2; ACTC; Actc-1; ACTC1; Acts; ACTSA; Actsk-1; ACTVS; alphaSMA; ASD5; ASMA; BOS_10523; BOS_23286; BOS_23966; CFTD; CFTD1; CFTDM; CMD1R; CMH11; GIG46; LVNC4; MPFD; MYMY5; NEM1; NEM2; NEM3; SHPM; SMalphaA
UniProt ID: (Human) P68133, (Bovine) P68138, (Pig) P68137, (Human) P62736, (Bovine) P62739, (Human) P68032, (Bovine) Q3ZC07, (Rat) P68136, (Mouse) P68134, (Rat) P62738, (Mouse) P62737, (Mouse) P68033, (Rat) P68035
Entrez Gene ID: (Human) 58, (Bovine) 281592, (Dog) 488984, (Pig) 100154254, (Human) 59, (Bovine) 515610, (Human) 70, (Bovine) 533219, (Rat) 29437, (Mouse) 11459, (Rat) 81633, (Mouse) 11475, (Mouse) 11464, (Rat) 29275
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