|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2-4 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Western Blot (WB)||2 µg/ml|
|Miscellaneous PubMed (MISC)||See 2 publications below|
|Western Blot (WB)||See 6 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Rabbit|
|Published species reactivity||Rabbit , Human Mouse|
|Host / Isotype||Mouse / IgG|
|Immunogen||Full length, recombinant, human adiponectin.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-054 detects adiponectin from human, rabbit and mouse samples.
MA1-054 has been used successfully in Western blot, immunofluorescence, and ELISA procedures. By Western blot this antibody detects ~30 kDa protein representing Adiponectin in 3T3-L1 adipocytes. Immunofluorescence and ELISA of Adiponectin can also be performed using MA1-054.
The MA1-054 immunogen is full length, recombinant, human adiponectin.
Adiponectin is a protein hormone secreted exclusively by adipocytes that regulates the metabolism of lipids and glucose. Adiponectin has been shown to play a role in various physiological processes including homeostasis and obesity. Decreased levels of adiponectin are associated with obese individuals as well as insulin resistance and hyperinsulinemia (type 2 diabetes). In mice adiponectin has been shown to improve insulin signaling, thus ameliorating insulin resistance. Through activation of AMP kinase and PPAR-alpha, adiponectin can increase fatty acid transport, oxidation, and dissipation in skeletal muscle, leading to a reduction of intramyocellular lipid levels and thus improved insulin signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 30 kDa adipocyte complement-related protein; a; ACRP30; Adipocyte; adipocyte complement related protein; Adipocyte complement-related 30 kDa protein; adipocyte, C1Q and collagen domain containing; Adipocyte, C1q and collagen domain-containing protein; Adipocyte-specific protein AdipoQ; Adiponectin; Adipose most abundant gene transcript 1; Adipose most abundant gene transcript 1 protein; adipose specific collagen-like factor; apM-1; C1q and collagen domain-containing protein; Gelatin-binding protein; gelatin-binding protein 28
Gene Aliases: 30kDa; ACDC; ACRP30; Ad; adipo; ADIPOQ; ADIPQTL1; ADPN; APM-1; APM1; APN; GBP28
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