|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||5 µg/ml|
|Immunofluorescence (IF)||5 µg/ml|
|Immunoprecipitation (IP)||Assay Dependent|
|Radioimmune Assays (RIA)||Assay Dependent|
|Western Blot (WB)||1 µg/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified AFP from Cord Blood|
|Storage buffer||0.01M potassium phosphate with 2.5mM KCl, 0.135M NaCl, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
MA5-14665 can be used for immunofluorescent analysis of AFP in the endoderm derived from human embryonic stem cells. By Western blot, MA5-14665 detects endogenous AFP protein in the early hepatocyte-like cells derived from human embryonic stem cells.
Product MA514665 is a smaller package size of MIA1301 (formerly sold as a Seradyn product).
This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Alpha-1-fetoprotein; Alpha-fetoglobulin; Alpha-fetoprotein
Gene Aliases: AFP; AFPD; FETA; HPAFP
UniProt ID: (Human) P02771
Entrez Gene ID: (Human) 174
Molecular Function: transfer/carrier protein
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