|Western Blot (WB)||1:2000|
|Tested Species reactivity||Many|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant rat alpha-internexin expressed in and purified from E. coli|
|Storage buffer||tissue culture supernatant|
|Contains||10mM sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
In Western blot, a band at ~66 kDa is detected. The epitope recognized by MA1-4709 (C-terminal non-helical extension of the protein) is unusually resistant to aldehyde fixation, so formalin fixation of histological sections is recommended.
Alpha internexin is a Class IV intermediate filament originally discovered as it copurifies with other neurofilament subunits. On SDS-PAGE gels it runs with an apparent molecular weight of 64 to 66 kDa, with some species variability, although the real molecular weight is about 55kDa; as with the other neurofilament subunits the presence of highly negatively charged sequences results in reduction of SDS-PAGE mobility. a-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons a-internexin is the only neurofilament subunit expressed. Antibodies to a-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition, the very early developmental expression of a-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cells committed to the neuronal lineage. In addition, recent studies show a marked up-regulation of a-internexin during neuronal regeneration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 66 kDa neurofilament protein; Alpha-internexin; Alpha-Inx; FLJ18662; FLJ57501; internexin neuronal intermediate filament protein, alpha; internexin, alpha; MGC12702; NEF5; Neurofilament 5; neurofilament 5 (66kD); neurofilament-66; neurofilament-66, tax-binding protein; NF-66; TXBP-1
Gene Aliases: AV028420; INA; Inexa; Intlaa; NEF5; NF-66; NF66; TXBP-1
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