|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Western Blot (WB)||1:25|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to N-terminus of human Annexin VI.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is liver tissue.
The Annexin family of calcium-binding proteins is composed of al least ten mammalian genes. It is characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. The Annexin family has been implicated as regulators of such diverse processes as ion-flux, endocytosis and exocytosis, and cellular adhesion. When overexpressed in A431 cells, Annexin VI causes a partial reversal of the transformed phenotype.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 67 kDa calelectrin; Annexin A6; Annexin VI; annexin VI (p68); annexin VI p68; Annexin-6; ANX6; calcium-binding protein p68; calelectrin; calphobindin II; Calphobindin-II; CBP68; Chromobindin-20; CPB-II; Lipocortin VI; p68; p70; Protein III; testis secretory sperm-binding protein Li 198a
Gene Aliases: ANX6; ANXA6; CBP68
UniProt ID: (Human) P08133
Entrez Gene ID: (Human) 309
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