|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Immunoprecipitation (IP)||Assay Dependent|
|Radioimmune Assays (RIA)||Assay Dependent|
|Western Blot (WB)||1:500-1:2000|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified human plasma Apo A-1|
|Storage buffer||0.015M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.1% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
By sandwich ELISA, MIA1404 can be used as a detection antibody with Product # 710263 or # 701239 as a coating antibody, to generate a matched pair. Using these matched pairs, recombinant human Apo A-1, but not recombinant mouse Apo A-1, was detected. MIA1404 can be used to detect Apo A-1 from serum samples. To increase sensitivity of sandwich ELISAs with MIA1404, a biotinylated detection antibody followed by Streptavidin-HRP is recommended.
By Western blot, MIA1404 detects recombinant human Apo A-1, but not recombinant mouse Apo A-1. MIA1404 is also recommended for detecting endogenous Apo A-1 by Western blot.
MIA1404 was formerly sold as a Seradyn product.
This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Apo-AI; apoA-I; Apolipoprotein A-I; Apolipoprotein A1
Gene Aliases: Alp-1; apo(a); apo-AI; Apoa-1; apoA-I; APOA1; Brp-14; Ltw-1; Lvtw-1; Sep-1; Sep-2; Sep2
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