|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunoprecipitation (IP)||Assay Dependent|
|Radioimmune Assays (RIA)||Assay Dependent|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Purified human serum LDL|
|Storage buffer||0.01M potassium phosphate with 0.135M NaCl, 2.5mM KCl, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
MA5-14740 targets Apolipoprotein B in ELISA, IP, and RIA applications and shows reactivity with Human samples.
The MA5-14740 immunogen is purified human serum LDL.
MA5-14740 detects Apolipoprotein B which has a predicted molecular weight of approximately 513 kDa.
Product MA514740 is a smaller package size of MIA1606 (formerly sold as a Seradyn product).
This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Apo B-100; apoB-100; apoB-48; apolipoprotein B (including Ag(x) antigen); Apolipoprotein B-100; apolipoprotein B47; apolipoprotein B48
Gene Aliases: APOB; apoB-100; apoB-48; FLDB; LDLCQ4
UniProt ID: (Human) P04114
Entrez Gene ID: (Human) 338
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