|Immunohistochemistry (Frozen) (IHC (F))||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:200|
|Western Blot (WB)||1:1000-1:5000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide made to a C-terminus portion of the rat protein (within residues 200-300).|
|Purification||Antigen affinity chromatography|
|Contains||0.05% sodium azide|
|Storage conditions||4° C, do not freeze|
In Western blot, bands can be seen ~28 kDa for the non-glycosylated form and ~37 kDa for the glycosylated form.
Suggested positive control: rat kidney, renal inner medulla.
Aquaporin 2 (AQP2) is a membrane protein involved in plasma membrane water transport in the renal collecting duct. This antibody is targeted to a peptide sequence upstream from the phosphorylated region that, upon phosphorylation, can diminish the antibody signal in immunoblots and immunocytochemistry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ADH water channel; AQP-2; AQP-CD; aquaporin 2 (collecting duct); Aquaporin-2; Aquaporin-CD; Collecting duct water channel protein; Water channel protein for renal collecting duct; water-channel aquaporin 2; WCH-CD
Gene Aliases: AQP-2; AQP-CD; AQP2; aquaporin-2; cph; jpk; WCH-CD
Molecular Function: transporter
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