|Immunofluorescence (IF)||1:100 - 1:1000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10 - 1:100|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500 - 1:2000|
|Western Blot (WB)||See 19 publications below|
|ChIP assay (ChIP)||See 2 publications below|
|ELISA (ELISA)||See 2 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Pig , Non-human primate , Human Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of human AhR.|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-514 detects the aryl hydrocarbon receptor (AhR) from human, mouse, and rat cells.
MA1-514 has been successfully used in Western blotting, immunoprecipitation, immunofluorescence and immunohistochemistry procedures. By Western blot, this antibody detects an ~96 kDa protein representing AhR, with some minor non-specific bands observed in certain samples.
MA1-514 immunogen is residues 12-31 from the human AhR protein with amino acids 18-21 being omitted.
The aryl hydrocarbon receptor (AhR), also known as the dioxin receptor, is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AhR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for the Ah Receptor.
Studies indicate that in non-ligand activated cells, AhR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form. This transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). The AhR-ligand complex appears to initiate gene transcription of cytochrome P450 1A1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Ah receptor; AH-receptor; AHR; aromatic hydrocarbon receptor; Aryl hydrocarbon receptor; BHLHE76; Class E basic helix-loop-helix protein 76; dioxin receptor
Gene Aliases: Ah; Ahh; AHR; Ahre; BHLHE76; In
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