|ChIP assay (ChIP)||1:100|
|Flow Cytometry (Flow)||1:50|
|Gel Shift (GS)||Assay dependent|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500 - 1:2000|
|Immunoprecipitation (IP)||See 2 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Western Blot (WB)||See 5 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Gel Shift (GS)||See 3 publications below|
|ChIP assay (ChIP)||See 3 publications below|
|ELISA (ELISA)||See 2 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat , Human Mouse|
|Host / Isotype||Mouse / IgG3, kappa|
|Immunogen||Synthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of mouse AhR.|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-513 detects the aryl hydrocarbon receptor (AhR) from human, mouse, and rat cells.
MA1-513 has been successfully used in immunoprecipitation, chromatin immunoprecipitation, immunocytochemistry, immunohistochemistry, immunofluorescence, and gel shift procedures. This antibody specifically immunoprecipitates a single ~95 kDa protein representing AhR from Hepa 1 cytosol. Although MA1-513 can be used in Western blotting procedures, MA1-514 (clone RPT1) is recommended for this procedure.
The MA1-513 immunogen is a synthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of mouse AhR.
The aryl hydrocarbon receptor (AhR), also known as the dioxin receptor, is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AhR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for the Ah Receptor.
Studies indicate that in non-ligand activated cells, AhR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form. This transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). The AhR-ligand complex appears to initiate gene transcription of cytochrome P450 1A1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Ah receptor; AH-receptor; AHR; aromatic hydrocarbon receptor; Aryl hydrocarbon receptor; BHLHE76; Class E basic helix-loop-helix protein 76; dioxin receptor
Gene Aliases: Ah; Ahh; AHR; Ahre; BHLHE76; In
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