|Flow Cytometry (Flow)||3-5 µg/million cells|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
|Western Blot (WB)||See 1 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Bovine , Sheep , Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the N-terminal region of the human Aurora B protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
Aurora-B kinase (AurB) is a serine/threonine kinase which is the enzymatic core of the Chromosomal Passenger Complex (CPC). This complex functions in chromosome alignment and separation, histone modification, and cytokinesis. CPC also includes three non-enzymatic subunits known as the inner centromere protein (INCENP), survivin, and borealin, which determine the activity, localization, stability, and possibly even the substrate specificity of AurB. Two CPCs exist during mitosis: one containing all four members and another consisting of INCENP and AurB. Quaternary CPC functions during chromosome alignment and cytokinesis, whereas the INCENP-AurB complex may be responsible for modifying histone H3. AurB is overexpressed in a number of tumours, including primary breast and colon tumour samples. Its expression in tumours seems to be linked with Aurora-A kinase, suggesting a feedback mechanism between the two proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AIK2; AIM-1; AIM1; ARK-2; ARK2; AURKB; Aurora 1; Aurora kinase B; aurora kinase B-Sv1; aurora kinase B-Sv2; Aurora- and IPL1-like midbody-associated protein 1; aurora-1; Aurora-B; Aurora-related kinase 2; Aurora/IPL1-related kinase 2; EC 22.214.171.124; protein phosphatase 1, regulatory subunit 48; serine/threonine kinase 12; Serine/threonine protein kinase 12; Serine/threonine-protein kinase 12; Serine/threonine-protein kinase 5; Serine/threonine-protein kinase aurora-B; STK-1; STK12
Gene Aliases: AIK2; AIM-1; AIM1; AIRK2; ARK2; AurB; AURKB; aurkb-sv1; aurkb-sv2; IPL1; PPP1R48; STK1; STK12; STK5
UniProt ID: (Human) Q96GD4
Entrez Gene ID: (Human) 9212
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.