|Flow Cytometry (Flow)||1/25 - 1/50|
|Immunohistochemistry (Frozen) (IHC (F))||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1/50|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS, pH 7.4|
|Contains||0.09% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul. A suggested positive control for immunohistochemical applications is tonsil or breast carcinoma. This product does not require protein digestion pre-treatment of paraffin embedded sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.
B7-H4, also known as B7x, a costimulatory protein which is reported to function as a negative regulator of T-cell mediated immunity. Although B7-H4 binds an unknown receptor, it is thought to deliver an inhibitory signal to T-cells preventing their proliferation, cell cycle progression and interleukin-2 production. B7-H4 deficient mice are only minimally affected; suggesting B7-H4 is important in the fine tuning of the T-cell mediated immune response.
B7-H4 is expressed on activated T-cells, B-cells, monocytes and dendritic cells. Aberrant expression has been associated with cancers of the lung, breast and ovary in humans. B7-H4 could be a useful prognostic marker in Renal Cell Carcinoma (RCC).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B7 family member, H4; B7 homolog 4; B7 superfamily member 1; B7-H4; B7h.5; B7H4; B7S1; B7X; FLJ22418; Immune costimulatory protein B7-H4; PRO1291; Protein B7S1; RP11-229A19.4; T cell costimulatory molecule B7x; T-cell costimulatory molecule B7x; V-set domain-containing T-cell activation inhibitor 1; VCTN1; VTCN1
Gene Aliases: B7-H4; B7h.5; B7H4; B7S1; B7X; PRO1291; UNQ659/PRO1291; VCTN1; VTCN1
UniProt ID: (Human) Q7Z7D3
Entrez Gene ID: (Human) 79679
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