|Immunohistochemistry (Paraffin) (IHC (P))||2-10 ug/ml|
|Tested Species reactivity||Human, Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region near the N-terminus of human BACE1, conjugated to KLH.|
|Contains||0.1% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
Predicted to react with bovine, hamster and mouse based on sequence homology.
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. A suggested positive control for immunohistochemical applications is Alzheimer's affected brain tissue.
B-site amyloid precursor protein (APP)-cleaving enzyme 1 (BACE1) is a 75kD type I integral membrane glycoprotein and aspartic protease. BACE1 is a member of the peptidase A1 protein family, and is expressed by neurons and astrocytes in the brain. BACE1 catalyzes the cleavage of beta APP at the extracellular, amino-terminal domain leading to the formation of amyloid beta peptide. Amyloid beta peptide is the major constituent of amyloid plaques in the brains of individuals afflicated with Alzheimer's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: APP beta-secretase; asp 2; ASP2; Aspartyl protease 2; BACE; Beta-secretase 1; beta-secretase 1 precursor variant 1; beta-site amyloid beta A4 precursor protein-cleaving enzyme; Beta-site amyloid precursor protein cleaving enzyme 1; beta-site APP cleaving enzyme 1; beta-site APP-cleaving enzyme 1; FLJ90568; HSPC104; KIAA1149; Memapsin-2; Membrane-associated aspartic protease 2; transmembrane aspartic proteinase Asp2
Gene Aliases: ASP2; BACE; BACE1; HSPC104; KIAA1149
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