|Immunohistochemistry (Paraffin) (IHC (P))||1:300|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from N-terminus of human BAK.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is tonsil tissue.
BAK or BCL-2-homologous antagonist is a member of the BCL-2 family of homologous proteins which function in the regulation of apoptosis. The BCL-2 related proteins interact with one another through the formation of homo- and heterodimers. Bak has been localized by immunohistochemistry to the cytosol of cells and stains in a punctate pattern characteristic of other BCL-2 family proteins, which have been localized to intracellular organelles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: apoptosis regulator BAK; BAK; BAK-LIKE; bcl-2 homologous antagonist/killer; bcl-2-like protein 7; bcl2-L-7; BCL2-like 7 protein; BCL2L7; CDN1; pro-apoptotic protein BAK
Gene Aliases: BAK; BAK-LIKE; BCL2L7; CDN1
UniProt ID: (Human) Q16611
Entrez Gene ID: (Human) 578
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.