|Immunohistochemistry (Frozen) (IHC (F))||1:15-1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:15-1:50|
|Western Blot (WB)||1:100-1:750|
|Immunohistochemistry (IHC)||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant, peptide sequence corresponding to residues 41-54 of Bcl-2 oncoprotein.|
|Storage buffer||tissue culture supernatant diluted in 6M urea|
|Storage conditions||4° C, do not freeze|
MA1-26233 detects Bcl2 from human samples.
MA1-26233 has been successfully used in immunoprecipitation, Immunohistochemistry, and Western blot procedures. Positive control of Tonsil or human lung cell line suggested. This antibody works well with B5 or Bouin's fixed tissues. Formalin fixed tissues may give inconsistent results.
The MA1-26233 immunogen is recombinant, peptide sequence corresponding to residues 41-54 of Bcl-2 oncoprotein.
Bcl2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of Bcl2, such as in the case of translocation of Bcl2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; Bcl-2; protein phosphatase 1, regulatory subunit 50
Gene Aliases: Bcl-2; BCL2; PPP1R50
UniProt ID: (Human) C9JHD5
Entrez Gene ID: (Human) 596
Molecular Function: signaling molecule
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