|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to N-terminus of human BCL-2 alpha protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.
Expression of BCL-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; Bcl-2; PPP1R50; protein phosphatase 1; protein phosphatase 1, regulatory subunit 50; regulatory subunit 50
Gene Aliases: Bcl-2; BCL2; PPP1R50
UniProt ID: (Human) C9JHD5
Entrez Gene ID: (Human) 596
Molecular Function: signaling molecule
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