|Flow Cytometry (Flow)||1 ug/test|
|Immunohistochemistry (Paraffin) (IHC (P))||1:400|
|Immunoprecipitation (IP)||2-4 ug|
|Immunohistochemistry (IHC)||See 11 publications below|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Human Mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||A synthetic peptide corresponding to aa 35-50 (TEAPEETEAERETPSA) of mouse Bcl-xL protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-11950 targets BCL-XL in FACS, IF, IHC (P) and IP applications.
MA5-11950 can be used to stain cells by IF following fixation/permeabilization in either methanol or 4% paraformaldehyde/0.5% octyl-beta-glucoside. The antibody can be used at a lower concentration when fixed and permeabilized by the latter method.
Bcl-X has two isoforms, Bcl-XL (long) a 241 amino acid protein and Bcl-XS (short) a 178 amino acid protein lacking a 63 amino acid domain that is well conserved among members of the Bcl-2 family. Bcl-XL represses cell death, while Bcl-XS favors cell death.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: anti-apoptosis regulatory protein; Apoptosis regulator Bcl-X; B-cell leukemia/lymphoma x; Bcl-2-like protein 1; Bcl2-L-1; BCL2-like 1; protein phosphatase 1, regulatory subunit 52
Gene Aliases: Bcl(X)L; Bcl-X; Bcl-XL; BCL-XL/S; bcl2-L-1; BCL2L; BCL2L1; BCLX; PPP1R52
Molecular Function: signaling molecule
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