|ELISA (ELISA)||1-10 µg/ml|
|Inhibition Assays (IA)||1-10 µg/ml|
|Immunohistochemistry (IHC)||1-10 µg/ml|
|Western Blot (WB)||1-10 µg/ml|
|Tested Species reactivity||Human, Rat|
|Host / Isotype||Sheep / IgG|
|Immunogen||Recombinant human BDNF.|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.|
By ELISA, this antibody exhibits a cross-reactivity of less than 1% to recombinant human BDNF, NT3 and NT4/5.
Use at 1-10µg/ml for inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10µg/ml (ED50).
Reconstitute with 500 ul of distilled water. After reconstitution keep aliquots at -20° for a higher stability, and at 4°C with an appropriate antibacterial agent.
The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Abrineurin; BDNF; brain derived neurothrophic factor; brain derived neurotrophic factor; Brain-derived neurotrophic factor; neurotrophin
Gene Aliases: ANON2; BDNF; BULN2
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