|Flow Cytometry (FACS)||Neat-1:10|
|Immunofluorescence (IF)||Assay Dependent|
|Tested Species reactivity||Mouse|
|Host / Isotype||Hamster / IgG|
|Immunogen||A complex of mouse BIM and Bcl-XL produced in insect cells.|
|Storage buffer||PBS with 1% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
Membrane permeabilization is required for flow cytometry applications. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
Bcl-2 (B-cell lymphoma) and close homologues promote cell survival while many of its relatives lead to apoptotic cell death. The Bcl-2 homology 3 (BH3) domain is a potent death domain, and a novel BH3 domain containing protein, termed Bim or BOD (Bcl-2-related Ovarian Death Gene), has recently been cloned. Bim possesses a hydrophobic C-terminus and is localized to the intracytoplasmic membranes. Bcl-2 binds to Bim and modulates its death function, while Bcl-2 mutants are unable to do either.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: bcl-2-like protein 11; Bcl2 interacting mediator of cell death; bcl2-interacting mediator of cell death
Gene Aliases: 1500006F24Rik; bcl2-L-11; Bim; Bod
UniProt ID: (Mouse) O54918
Entrez Gene ID: (Mouse) 12125
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