|Immunocytochemistry (ICC)||5 µg/ml|
|Immunofluorescence (IF)||5 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Western Blot (WB)||2.0-3 µg/ml|
|Tested Species reactivity||Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (291 STMNGTSNSPSGNHQSS 307) of BMI1 protein.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
BMI1 is a 326 amino acid containing protooncogene belonging to the polycomb gene family with a RING-type zinc finger domain. A transcription factor and a senescence regulator, BMI1 is a component of the Polycomb group (PcG) multiprotein PRC1 complex, required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. An important role of BMI1 has been reported in regulating proliferation of stem cells and tumor cells in diverse adult epithelial tissues involved in human colorectal carcinogenesis by repressing the INK4a/ARF proteins (CDKN2A). This protein also plays an essential role in the generation of self-renewing adult hematopoietic stem cells and in the maintenance and expansion of immature granule cell precursors. BMI1 cooperates with the MYC oncogene to produce B-lymphomas. Ubiquitous expression is seen in most of the tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B lymphoma Mo-MLV insertion region 1; B lymphoma Mo-MLV insertion region 1 homolog; BMI1 polycomb ring finger oncogene; BMI1 polycomb ring finger proto-oncogene; flvi-2/bmi-1; FLVI2/BMI1; murine leukemia viral (bmi-1) oncogene homolog; PCGF4; Polycomb complex protein BMI-1; polycomb group protein Bmi1; polycomb group ring finger 4; Polycomb group RING finger protein 4; ring finger protein 51; RNF51; RP11-573G6.1
Gene Aliases: AW546694; Bmi-1; Bmi1; flvi-2/bmi-1; FLVI2/BMI1; PCGF4; RNF51
UniProt ID: (Mouse) P25916
Molecular Function: ubiquitin-protein ligase
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