|Immunohistochemistry (Paraffin) (IHC (P))||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1-10 µg/ml|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1, k|
|Immunogen||Protein fragment expressed in E. coli corresponding to amino acids 341-748.|
|Storage buffer||PBS, pH 7.2|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
MA1-23162 detects BRCA1 in Human and Mouse samples.
MA1-23162 has been successfully used in Immunohistochemistry, Immunoprecipitation and Western Blot procedures. For immunohistochemistry (paraffin), perform heat mediated antigen retrieval before commencing with IHC staining protocol (0.1M citrate buffer at 95°C in water bath for 20 minutes). Avoid nonspecific binding by incubation in normal rabbit anti-mouse serum. Incubate primary antibody overnight and use biotin-streptavidin amplification kit. Block endogenous peroxidase with 0.3% H2O2 for 30 minutes.
MA1-23162 immunogen corresponds to Protein fragment expressed in E. coli corresponding to amino acids 341-748..
BRCA1-8F7 recognizes full-length BRCA1, a 220kDa nuclear phosphoprotein. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Its usefulness to monitor functional inactivation of BRCA1 in sporadic breast cancers is under active investigation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: BRCA1/BRCA2-containing complex, subunit 1; breast and ovarian cancer susceptibility protein 1; breast and ovarian cancer sususceptibility protein 1; Breast Cancer 1; Breast Cancer 1 Early Onset; breast cancer 1, early onset; breast cancer associated 1; Breast cancer type 1 susceptibility protein; Breast cancer type 1 susceptibility protein homolog; Breast Ovarian Cancer Susceptibility; early onset breast cancer 1; Fanconi anemia, complementation group S; IRIS; Papillary Serous Carcinoma Of The Peritoneum; protein phosphatase 1, regulatory subunit 53; PSCP; RING finger protein 53; RING-type E3 ubiquitin transferase BRCA1; RNF53; truncated BRCA1; truncated breast and ovarian cancer susceptibility protein 1; truncated breast and ovarian cancer sususceptibility protein 1; truncated breast cancer type 1 susceptibility protein
Gene Aliases: BRCA1; BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4; PPP1R53; PSCP; RNF53
Molecular Function: ubiquitin-protein ligase
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