|Immunohistochemistry (Paraffin) (IHC (P))||1:400|
|Western Blot (WB)||1:10,000|
|Tested Species reactivity||Bovine, Dog, Human, Mouse, Non-human primate, Pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide made to an internal portion (residues 150-300) of human Beclin-1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.1% BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
Suggested positive control: HeLa whole cell extract, antigen standard for BECN1 (transient overexpression lysate).
Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic-lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells.
Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl-2-binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 60 kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons. In gene transfer studies, beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammary tumorigenesis, and inhibits viral replication. Expression of the Beclin 1 protein is frequently decreased in malignant breast epithelial cells.
Based upon these observations, it is speculated that beclin 1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections. Beclin 1 may also play a role in other biologic processes in which autophagy is important such as cell differentiation and nutritional stress responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ATG6; ATG6 autophagy related 6 homolog; Bcl-2-interacting protein beclin; beclin 1 (coiled-coil, moesin-like BCL2-interacting protein); beclin 1, autophagy related; Beclin-1; beclin1; BECN1; Coiled-coil myosin-like BCL2-interacting protein; coiled-coil, myosin-like BCL2-interacting protein; GT197; Protein GT197; testis secretory sperm-binding protein Li 215e; VPS30
Gene Aliases: 4921513J16Rik; 5430417M23Rik; ATG6; beclin1; BECN1; BOS_19107; GT197; VPS30
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