|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||3 - 5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||1:1,000|
|Western Blot (WB)||See 13 publications below|
|Tested Species reactivity||Chicken, Human, Mouse, Rabbit, Rat|
|Published species reactivity||Rat , Human , Mouse , Guinea pig|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Beta-actin N-terminal peptide|
|Storage buffer||PBS, pH 7.2|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
MA5-15739 detects beta actin from human, mouse, rat, rabbit, and chicken samples.
MA5-15739 has been successfully used in Western blot, FACS, and ELISA.
MA5-15739 may be stored at 4°C short term. Add 0.05% sodium azide if desired. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Beta-actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: actin, beta; Actin, cytoplasmic 1; Atcb; beta cytoskeletal actin; Beta-actin; cytoplasmic 1; cytoplasmic beta-actin; gamma non-muscle actin; PS1TP5-binding protein 1
Gene Aliases: ACT-5; ACTB; actin; Actx; Bact; beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1; RCJMB04_4h19
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