|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||1 ug/test|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:500|
|Western Blot (WB)||1:2000 - 1:5000|
|Western Blot (WB)||See 25 publications below|
|Tested Species reactivity||Chicken, Human, Mouse, Non-human primate, Rabbit, Rat|
|Published species reactivity||Avian , Rat , Mouse , Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||KLH conjugated peptide of the N-terminus of Beta-Tubulin.|
|Storage buffer||PBS, pH 7.2|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA5-16308 detects beta-tubulin in human, monkey, mouse, rat, rabbit and chicken samples with ELISA, FACS, IHC (P), Western blot and immunofluorescence applications.
Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: beta 5-tubulin; beta Ib tubulin; beta polypeptide; beta-4 tubulin; c(beta)7 tubulin; tubulin; Tubulin beta 4'; Tubulin beta chain; tubulin beta polypeptide; Tubulin beta-1 chain; Tubulin beta-5 chain; Tubulin beta-7 chain; tubulin, beta 2; tubulin, beta 2A; tubulin, beta 2A class IIa; tubulin, beta class I; tubulin, beta polypeptide
Gene Aliases: 2810484G07Rik; CDCBM6; CSCSC1; M(beta)1; M40; OK/SW-cl.56; TUBB; TUBB1; TUBB2A; TUBB5
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