|Immunohistochemistry (IHC)||5 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1, Kappa|
|Immunogen||A full-length human Bif-1 recombinant protein was used as immunogen.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
The Bcl-2 family consists of anti-apoptotic (or cell survival) genes, such as Bcl-2, Bcl-XL and pro-apoptotic (or cell death) genes including Bax and BAD. In addition to its anti-apoptotic effect, Bcl-2 also inhibits progression through the cell cycle. Functional interactions between family members as well as binding to other cellular proteins modulate their activities. Bcl-2 family members are important in normal tissue development, homeostasis, and disease states. Bcl-2 prevents cell death by forming heterodimers with Bax and Bad (1). Bax promotes cell death by forming homodimers, which can be blocked by forming heterodimers with Bcl-2 or BCL-XL. Recently, using yeast two-hybrid system Cuddeback et al (product citation 2) have identified a cDNA encoding a 365 amino acid protein, which interacts physically with Bax protein and influences cell life and death. Bif-1 contains a Src homology 3 (SH3) domain in the C-terminus. It does not contain any of the conserved Bcl-2 homology (BH) domains of the Bcl-2 family proteins. Human Bif-1 protein shares 96% and 42% identity at the amino acids level with mouse and C. elegans, respectively. Overexpression of Bif-1 in transfected cell lines promotes Bax conformational change, caspase activation, and apoptotic cell death following growth factor withdrawal. Bif-1 RNA is abundantly expressed in heart, skeletal muscle, kidney, and placenta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Bax-interacting factor 1; Bif-1; CGI-61; dJ612B15.2; Endophilin-B1; PPP1R70; protein phosphatase 1; protein phosphatase 1, regulatory subunit 70; regulatory subunit 70; SH3 domain-containing GRB2-like protein B1; SH3-containing protein SH3GLB1; SH3-domain GRB2 like endophilin B1; SH3-domain GRB2-like endophilin B1; testicular tissue protein Li 172
Gene Aliases: Bif-1; CGI-61; dJ612B15.2; KIAA0491; PPP1R70; SH3GLB1
UniProt ID: (Human) Q9Y371
Entrez Gene ID: (Human) 51100
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