|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Western Blot (WB)||1-4 µg/ml|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 15 amino acid peptide from near the amino-terminus of human Bit1.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
A suggested positive control is A-20 cell lysate.
PA5-20167 can be used with blocking peptide PEP-0284.
Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apoptosis induced by integrin detachment. Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process. Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE. Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Bcl-2 inhibitor of transcription; Bcl-2 inhibitor of transcription 1; CGI-147, BIT1, PTH2; cilia and flagella associated protein 37; Peptidyl-tRNA hydrolase 2, mitochondrial; PTH 2
Gene Aliases: A230072I16Rik; BIT1; CFAP37; CGI-147; IMNEPD; PTH; PTH 2; PTH2; PTRH2; RGD1306819
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