|Western Blot (WB)||0.5-2 µg/ml|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide made to an internal region (within residues 1-100) of the human Bmi1 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||sodium borate|
|Storage conditions||4° C, store in dark|
Suggested positive control: human and mouse skeletal muscle.
The Bmi1 oncogene has been shown to induce telomerase activity and immortalize human mammary epithelial cells. Bmi1 extends the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway. Bmi1 is also important in maintaining stem cells' ability to self renew. It has also been termed an oncogene with aberrant expression in various carcinomas. It has been shown to be a negative regulator of p19Arf, Ink4a (p14Arf) and tp53 known tumor repressors. Ablation of Bmi1 is required for short term survival of cancer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B lymphoma Mo-MLV insertion region 1; B lymphoma Mo-MLV insertion region 1 homolog; BMI1 polycomb ring finger oncogene; BMI1 polycomb ring finger proto-oncogene; flvi-2/bmi-1; FLVI2/BMI1; murine leukemia viral (bmi-1) oncogene homolog; PCGF4; Polycomb complex protein BMI-1; polycomb group protein Bmi1; polycomb group ring finger 4; Polycomb group RING finger protein 4; ring finger protein 51; RNF51; RP11-573G6.1
Gene Aliases: AW546694; Bmi-1; Bmi1; flvi-2/bmi-1; FLVI2/BMI1; PCGF4; RNF51
UniProt ID: (Mouse) P25916
Molecular Function: ubiquitin-protein ligase
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.