|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:500|
|Immunocytochemistry (ICC)||See 17 publications below|
|Immunohistochemistry (IHC)||See 42 publications below|
|Tested Species reactivity||Chemical|
|Published species reactivity||Rat , Mouse , Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Bromodeoxyuridine (BrdU) conjugated to BSA|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-11285 targets Bromodeoxyuridine in FACS, IF, and IHC (P) applications and shows reactivity with All samples.
The MA5-11285 immunogen is bromodeoxyuridine (BrdU) conjugated to BSA.
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. BrdU can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 5-Bromo-2-deoxyuridine; Bromodeoxyuridine
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