|Flow Cytometry (Flow)||1:100 - 1:200|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:100-1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:200|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||Chemical|
|Published species reactivity||Hamster|
|Host / Isotype||Mouse / IgG1|
|Contains||0.09% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
OMA1-06200 detects bromodeoxyuridine (BrdU).
OMA1-06200 has been successfully used in immunocytochemistry, immunohistochemical and FACS procedures. This antibody specifically detects free BrdU and BrdU that has been incorporated into nuclear DNA.
OMA1-06200 antigen is purified BrdU.
Store at 4°C, or in small aliquots at -20°C.
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. BrdU can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 5-Bromo-2-deoxyuridine; Bromodeoxyuridine
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.