|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from C-terminus of human C4d|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.
C4 plays a central role in the activation of the classical pathway of the complement system. Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: basic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3; C4B1; C4B12; C4B2; C4B3; C4B5; C4F; CH; Chido form of C4; CO4; complement C4B1a; CPAMD3; DADB-112B14.11; FLJ60561; MGC164979
Gene Aliases: C4B1; C4B12; C4B2; C4B3; C4B5; C4B_2; C4BD; C4F; CH; CO4; CPAMD3
Entrez Gene ID: (Human) 721
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