|Immunohistochemistry (Frozen) (IHC (F))||1:200-1:1000|
|Western Blot (WB)||1:2000|
|Tested Species reactivity||Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant, full length rat protein.|
|Contains||0.1% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
PA1-22488 detects MCP1 in mouse and rat samples.
PA1-22488 has been successfully used in ICC/IF, IHC (F) and Western blot procedures.
The PA1-22488 immunogen is recombinant, full length rat protein.
Rat MCP-1/JE (monocyte chemoattractant protein-1) is a 148-amino acid CC chemokine with a NH2-terminal sequence of 29 residues as a signal sequence. It was originally cloned from Con A-stimulated rat spleen cDNA library. This rat MCP-1/JE is 49-amino acid longer than humanMCP-1 at 3'-end. This 3'-end is a serine and threonine rich zone, which is probably responsible for the extensive O-glycosylation and explains for the higher molecular weight (25 kDa). In vitro, MCP-1/JE is chemotactic for monocytes as well as lymphocytes and basophils, but not for neutrophils. MCP-1/JE is produced by a wide range of cell types as a reaction to diverse inflammatory stimuli.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AI323594; C-C motif chemokine 2; immediate-early serum-responsive JE protein; Immediate-early serum-responsive protein JE; JE; MCP-1; MCP1; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic protein 1; Platelet-derived growth factor-inducible protein JE; RP23-350G1.3; Scya2; Sigje; small inducible cytokine A2; small inducible gene JE; Small-inducible cytokine A2; SMC-CF
Gene Aliases: AI323594; Ccl2; HC11; JE; MCAF; MCP-1; MCP1; Scya2; Sigje; SMC-CF
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