|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||0.05-0.5 ug/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This antibody is predicted to react with Rhesus monkey and porcine based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Monocyte chemotactic protein-1 (MCP-1) also known as CCL2 has a predicted molecular weight of ~11kDa; however, it may run at a higher molecular weight due to post translational modifications such as glycosylation. MCP-1 is a member of cytokine family and plays an important role in immunoregulation and inflammatory processes. MCP-1 is indicated in the etiology of several immune disorders. MCP-1 is also linked with tumor progression and related angiogenesis. MCP-1 displays selective chemotactic activity towards monocytes and basophils but not neutrophils or eosinophils. MCP-1 binds to chemokine receptors CCR2 and CCR4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: C-C motif chemokine 2; C-C motif ligand 2; chemokine (C-C motif) ligand 2; GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; SCYA2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; Small-inducible cytokine A2; SMC-CF
Gene Aliases: CCL2; GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; SCYA2; SMC-CF
UniProt ID: (Human) P13500
Entrez Gene ID: (Human) 6347
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