|Flow Cytometry (Flow)||0.5-1 ug/test|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100 - 200|
|Western Blot (WB)||1-2 µg/ml|
|Flow Cytometry (Flow)||See 4 publications below|
|Immunohistochemistry (IHC)||See 10 publications below|
|Immunocytochemistry (ICC)||See 5 publications below|
|Blocking Assay (BLOCK)||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Bovine, Human|
|Published species reactivity||Rabbit , Pig , Human , Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Purified GP160 from cell membrane glycoproteins of fresh non-T/non-B acute lymphoblastic leukemia cells|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-11854 targets CD105 in FACS, IF, IHC (P), and WB applications and shows reactivity with Human and Bovine samples.
The MA5-11854 immunogen is purified GP160 from cell membrane glycoproteins of fresh non-T/non-B acute lymphoblastic leukemia cells.
MA5-11854 was used in the immunofluorescence analysis to successfully detect CD105 in human mesenchymal stem cells derived from iPSCs.
CD105 or Endoglin or GP160 is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists as an O- and N-glycosylated homodimer. Up-regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation-associated endothelial cell marker. CD105 binds to TGF-Beta1 and Beta3 with high affinity but not TGF-Beta2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD105; CD105 antigen; END; Endoglin; ENG; HHT1; ORW; ORW1
Gene Aliases: END; ENG; HHT1; ORW1
UniProt ID: (Human) P17813
Entrez Gene ID: (Human) 2022
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