|Flow Cytometry (Flow)||Assay Dependent|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG1/IgG2b|
|Immunogen||human peripheral blood monocytes from a rheumatoid arthritis patient|
|Conjugate||FITC , PE|
|Storage buffer||PBS with 0.2% carrier protein|
|Contains||0.08% sodium azide|
|Storage conditions||4° C, do not freeze|
This antibody is a monoclonal mixture of FITC-conjugated CD45 (clone BHPT-1, isotype IgG1) and PE-conjugated CD14 (clone FWKW, isotype IgG2b).
CD14 is a single-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS)-dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram-negative shock. CD45 Leukocyte Common Antigen (LCA) belongs to the family of at least four isoforms of membrane glycoproteins (220, 205, 190, 180 kDa) expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. The intracellular portion of these molecules have protein phosphatase activity and are involved in regulation of transmembrane signals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B220; c polypeptide; CD14; CD45; CD45 antigen; CD45R; GP180; L-CA; LCA; Leukocyte common antigen; LY5; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; protein tyrosine phosphatase; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; receptor type; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen
Gene Aliases: B220; CD14; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200
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