|Flow Cytometry (Flow)||1:10 -1:100|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:50-1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Immunohistochemistry (IHC)||See 2 publications below|
|Tested Species reactivity||Rat|
|Published species reactivity||Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Rat Spleen cell homogenate|
|Contains||0.09% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
This product requires protein digestion pre-treatment of paraffin sections using trypsin or pronase. A suggested positive control for immunohistochemical applications is liver. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD163 antigen is a type I membrane protein also known as M130 antigen, Ber-Mac3, Ki-M8 or SM4. CD163 is restricted in its expression to the monocytic/macrophage lineage. It is present on all circulating monocytes and most tissue macrophages except those found in the mantle zone and germinal centers of lymphoid follicles, interdigitating reticulum cells and Langerhan's cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD163 antigen; hemoglobin scavenger receptor; macrophage-associated antigen; scavenger receptor cysteine-rich type 1 protein M130
Entrez Gene ID: (Rat) 312701
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