|Flow Cytometry (Flow)||1/50|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Flow Cytometry (Flow)||See 1 publications below|
|Tested Species reactivity||Rat|
|Published species reactivity||Rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Activated rat T helper cells.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.09% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
We recommend the use of fixation for optimal staining results. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD2 belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Morover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD2; CD2 antigen; erythrocyte receptor; LFA-2; LFA-3 receptor; lymphocyte-function antigen-2; OX-34 antigen; OX-45 surface antigen homolog to human T lymphocyte CD2 antigen; rosette receptor; sheep red blood cell receptor; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5
Gene Aliases: Cd2; CD2R; LFA-2; LFA2; OX-34; OX34
UniProt ID: (Rat) P08921
Entrez Gene ID: (Rat) 497761
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.